AIMS

• The choice of anesthesia for neonatal mice lacks a consensus and directly impacts animal welfare and research outcomes.
• The methods used until now to assess animal welfare do not provide a complete understanding of potential pain or discomfort experienced during anesthesia.
• We aim to compare hypothermia and isoflurane anesthesia in neonates to better understand their impact on animal welfare.
• Our goal is to find the best method to reduce the stress and/or pain experienced by neonates during anesthesia and therefore the severity degree of procedures.
• Determine the most humane anesthesia method for neonates to refine animal experiments and improve well-being.